Tobacco Documents Online

'I'ill•: ('c,uxCii. F0ir'I'ulcarc0 Fh:Sr:A uc•n--U.S.A., I:vc. July 7, 1976 MF,MORA'JDUM v TO: L'rs. Bing, Hockett, Pfeier, Semmers and Stone FROM; W. U. Gardnrr SUBJL'CT: Application 1092PL , Koji Yoshinaga, Pia.D., Laboratory of Human Reproduction and Reproductive Biology, Harvarc3;•ledical School, Boston. "Effects of Nicotine ors Pregnancy." T},rc•e-quarters of this request is for salaries including 60% of the app?icEnt's. He ic this year f+atting in more time on the oroject. At least or.e of the pencing prorosals hes not been func?ed. The budget seems reascnable zor the work prorosed. As planned the effect of nicotine on the first wee): of pregnancy was invcstigatea during thc first year. Adequate data were not obtained on al]l points but the year wa.s on]y abcdt one-half ove.r when the report was written. The major findings to date are `.hat nicotine -- 2.5 mq. subcutaneossly two times daily delayed mast phases of ear]y devclop::.rnt from caily blanto.ryst fo:-mation to implantation. Toy,:,ber witl: a pro»E•rly timed injection of 0.2 ug estradiol the sit(:s of itnplanii:i,):• i•:crc clu.m {-cd near the tube] end of the uterus. There was a signific<:nt rEC:ucticn in ti,; estre,gen lactoqenic ard fol.liclc• : timulating hormone levels in the nicotin--tr~µted prcgnant rats (4th day of pregnancy). He plans to -tu8,- the cffccts of CO on nregnancy as well d1aring the coming year. lie thinks t}.at this may act on the carbonic anhydrase whic': is found in the uterus end in ovarir.n hormone dcpondent.. CO may also reduce the P950s. In one exnerircrnt hn P.roi>ucc s to injcct 7.5 mq nicotine tartraie subcuta•,ceusly two timcs icily to detcrwii:•: tht, etfcct on the hoi7-:oiues in the hypothal.nu-Lituitary- ovan, s: -tcm. 'rhin rcc^:,5 tn bc a)arc•:, r3o^c. We wint tc, he cert.ai.n that lie will not be meac:urinj to>:;ci.ty r..thcr than crr:ific und-organ effects. Jt mie,ht l,e i:'orthw?:ile to thinl; F-bout t'ic effect ef nicotine on the AHH in ta,,e hlacent:>s and the effects of nicotine cn the Proteolyt.ic enzymes and on protcolytic inh;bitors in thp placenta. These have been followed iu man and the former in rats with PAi] as well. W. U. Gardner WhG:ek