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Y l 3th Intrrnalional Cancer Congress Official Abstract Form . ~. Temp. Abst. No. Yene. AW6 No. s cA•It~cnRIF AND SUBCLASSII•1 ATtONS C (Flu tn 0 r.nr eueeort ~d oee s~s eludneaurn onl up nt n2 n! N ~ ~ m nx trt In il 13 112 ns 07 L 09 lo nR 03 m 0 Gf7 ~ ~ m 11 12 13 14 IS 16 17 18 TIIE EFFECT OF EXPOSURE TO NHOLE CIGARETTE SMOKE ON SHORT-TEIt~f ENDPOINTS IN BC3F1/CUM MICE. C.J. Henryl• K.K. Kanagalingam , K.K, llwangl, M. Dinowitzi, R.A. Lubetl, W.C. Ha11I, R. Rasmussen2, 1.~. Benedict3, a~nd R.E. Kouril, 1Mierobiologieal Associates, Bethesda, MD 20816, 2University of California, Irvine, CA 92717, 3Children's Hospital, Los Angeles, CA 90027. IT ut Using the SEM IIB cigarette smoking system, "nose-only" 0 exposure of BC3F1/Cum mice to 2R1 cigarettP smoke (high tar, o! _ ~ high nicotine) and 3A1 cigarette smoke (high tar, low nicotine) IIL OS resulted in deposition of 100-•400 ug smoke particulates/mouse/ 1k. day, with '6905 of the deposition in the respiratory tract. 0~ nH Dv In I IS Deposition of smoke particulates in the lung of these mice re- sulted in: a) 2-4 fold increase in lung microsomal mono- oxygenases by 6 hrs post exposure, b) '~2 fold increase in lung 1: ornithine decarboxylase activity after 12 weelcs smoke exposure 1! (5 days/week), c) 10-20 fold increase in 3H-thymidine incorpo- 10 ration (i.e. DNA, synthesis rates) by 9 weeks of daily smoke exposure, d) ti309~ increase in lung/body weight ratios (and total lung protein) by I week smoke exposure, and e) '~2 fold increase in sister chromatid exchanges in bone marrow after 1 week smoke exposure. Exposure to 3A1, but not 2R1, cigarette smoke resulted in an ti507 decrease in pulmonary DNA repair capacity. Exposure to smoke from either the A or R series cigarettes for ti52 weeks failed to alter splenic B-cell re- x sponses (Jernc plaque assay). Thus, cigarette smoke induces both organ specific and systemic biological responses in a murine model system. These responses could form the basis o ~~ for comparing the potential effects of various cigarette types 02 and smoking conditions. 0 Supported by The Council for Tobacco Researcti-USA, Inc. 04 0S 01 02 03 01 OS 2 ~ 05 1 7 W 01 OR 0 02 03 10 04 II nc 12 ~ CG/ee O7 Oa m Ox A S 01 W ^ ID o~ • ~ c ~ os L ~ 0a a Ixl oB UM 0! PRESENTATION PREFERENCE (Filt in ®one box oolr} 0 9ide ~Poper CSdeotific FOm OSdeatific EehibR 7ue lo the eise and oomplesity of the prolpaem. final decisions nprding e11de or poater eeteiom 'RL BE I MADEATTNEDISCRETIONOFT,IE NATIONAL PROGRAM COMMRTEE. AUTHOR INFORMATION: Paper to be pea~sented by nr_ Rich~rd F Y'n,.rj- O o•ho is the rost author an this abatraet OR 40 who is one of the authon of thisobnraet.bot not the f4et authoe. Ac+se Iist the fuB naroe, addew, and telephome number of the author pteaentFng the piper,aad afeo of the author to whom all oonespondenoe relating to the abotracllhould be tant Pirrxntieg Anthoe: Dr Richard r_ 1•r,,,r9 Microbiological Associates 5221 River Ce Coecapw~db,gAutlhar:-• Dr. Richard E. Kouri ,~ . A~-~t~C bC~eta. 5lgnature of Cun esnding Aulhor Abstncte not euBable for d'aect pholorcptoductlon will be tttrped at Ihe nomNul eop to the Coneryond• iog Author of $10.00 4U.S. DOLLARS). . 5