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/ PROPOSED STUDIES Contract CTR-0030 TABLE OF CONTENTS ABBREhIATIONS 1. CHRONIC INHALATION STUDIES A. Introduction Nt10119264 ~Y.:y =:/;~. B. C TTt-IOIA. Chronic exposure of BC3F1/Cum mice to cigarette smoke. ~ CTR-101B. Chronic exposure of C3H/Anf~Cum mice to , ciqarette smoke. - , CTR-lO1C. UFronic exposure of DBAi2J mice to cigarette smoke. 1I. SUPPORT SERVICES A', Dosimetry oCn i i : 1 1 1. CTR-102. Deposition, dosimetry and chronic smoke exposure usin 2Rl ei4arettes com ared to 2AI cigarettes in BC3F1 Cum mice. 8 ` 2, CTR-103. Evaluation of SEM II animal containment capa- s E14 eIty using - C- cigarettes. 10 ~ f l:u. .' ..:n 3. CTR-104. Depositlon and ddsimetr of 14C-DTC-3A1 iec•c:- cigarette_smo e In um mice. '. 10 ~ 4. CTR-105. Compar.atiye pv3luation of de_oosition and C! aos imetr a ter`exposu exposure to -na tnTi nm or catec o in a smoKe aeroso . il B. Distribution of Cigarette Smoke Constituents i!'1-1. CTR-106, Autoradioqra hic analvsis of lunra tissue a te r expos u re o m i ce to 2-c 7jc•.; r2 t t2 smoke : 12 t;QAnecs~~ ~ /R3~1 . aa 'r 1 a 2. CTR-107. _3H-Thymidine labelina index (LI ) in lung. 1 iyer a er, or i n~`e arter ex osure to smo e.or treatment wit carcinoaen.(Dr, R. Rasreusszn) 14 3. CTR-108. The effect of cigarette smoke from different t es o ciqarettes on uimonar mixea unction oxiases 0 eve s in t ree strains or mI ce. C. Short-Term Assays Relating to Possible Initiation Events. 1. CTR-96. Inh*.bition of 0monar DNA reoair ca acit a ter ex osure to cr arette srnoke, r, d~ Q .-asmussen . 15 18

/ PROPOSED Contract I STUD I ES CTR-0030 HT®0119264 2. CTR-109. Determination of in vivo damage__i_me ar~te,~d .,~ by ciqarette smo<~ e exposure using a GNA_ amaqea•a ine e ution assa 19 3. CTR-110` Detection and uantitation of inetabolites in urine o smo e expose mice capa e or causing mutation Mact~ e aT tester strains,~l Characterization of ulmonar c tochrommes invo ve in smo e associate 0 in eution T5r; - ang . 22 Alterations in the immune res onse after ex osure to oiqarette srno e r. H. erscowitz . 23 in re strains o mice urin c ronic 1 . .. ' g 25 6. CTR-112, smoke D. Short-Term Assays Relating to Possible Promoting Events 1. CTR-113A. Comparison of smoke induced oDC in three ~ strains o mice. ~.01rh, NCTR-113g._ iscrimination etween ODC inducticn andL• 1 ~~~ 'AHH in uction y cigarette smoke. `~ 20_ 2, CTR1 lG. Evaluati on .or' focus enhancement ass;y. . 29 0 " °~ 1'L ' E. Aerosol Studies. ~ 1. introduction 31 R:4 0 1 i`, .r-4 , 2. CTR-115. Acute toxicit of aerosolized TPA in the in re strains of mice. 32 ~~. i ~a of aerosol ized TPA.32 nsaM)!s.rV 3. CTR-116, l~~osition and -- • LO `vi '-,~ -" nitiate lung carcinogenesis. 33 ~ CTR-1i), ®locheriicail response to aerosolized chemicals. ,'5. CTR-118. Ca acit of aerosolized TPA to romote BaP :~.TPA vr Evaluation of ada tation and stress in the i.. . - ~