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I I I I I I i I I I I I ! I I I i I #82 AUTHOR: OLSON, JACK DATE: 1985 TITLE: CHRONIC CIGARETTE SIDESTREAM SMOKE EXXPOSURE INCREASES RAT TRACHEA ORNITHINE DECARBOXYLASE ACTIVITY CITATION: LIFE SCIENCES 37: 2165-2171, 1985 STUDY DESIGN: Male Sprague-Dawley rats were randomly divided into 4 - 6 groups: room control; sham; mainstream smoke; and sides:ream smoke diluted to 10%, 25% or 50%.Rats subjected to smoke were exposed to smoke from University of Kentucky 2R1 Reference cigarette for 10 minutes daily, 7 time/week for 4, 8 or 20 weeks. SS smoked rats were exposed to SS smoke collected in a 21Emin air flow using a glass container placed over the burning end of the cigarette. A fraction of this air flow which contained sidestream smoke was diluted with fresh room air and continuously diverted to the rats (see above for dilutions). 25 hours after the last smoke exposure, the rats were killed and trachea and whole lungs excised. Trachea and lung ornithine decarboxylase (ODC) and s-densosyl-methionine decarboxylase (AdoMet-DC) were determined. RNDINGS/RESULTS: Daily exposure to 25% SS smoke for either 4 or 8 weeks significantly increased trachea ODC activity. Four weeks of MS smoke did not alter trachea ODC activity, but 8 weeks of daily MS smoke exposure significantly increased trachea ODC activity. Trachea AdoMet-DC activity was not altered by either SS or MS smoke exposure. Four or 8 weeks of Ss smoke exposure did not increase lung ODC activity, however 8 weeks of MS smoke exposure significantly increased lung ODC activity. Lung AdoMet-DC activity was not altered by either SS or MS smoke exposure. 20 weeks of exposure to either 10% or 25% or 50% SS smoke significantly increased trachea ODC activity as compared to sham rats, whereas AdoMet-DC activity was not changed by SS smoke, MS smoke did not increase either ODC or AdoMet-DC activity in the trachea. Neither MS nor 10%, 25% or 50% SS smoke exposure for 20 weeks altered lung ODC or AdoMet-DC activity in the lung compared to shams. CONCLUSIONS: The major finding of this was was the clear demonstration that SS smoke exposure consistently elevated trachea ODC activity, however it did not alter lung ODC activity. The impact of MS smoke on trachea ODC activity was not as consistent as SS smoke. MS smoke increased trachea and lung ODC activity after 8 weeks, but not after 4 or 20 weeks of smoke exposure. Neither MS or SS smoke did not increase lung or trachea AdoMet-DC activity. 0