Tobacco Documents Online

! I I I I I I I I I I I I I I I I I # 100 AUTHOR: THOMAS, W. R., P.G. HOLT, J.M. PAPADIMITRIOU, AND D. KEAST DATE: 1974 TITLE: THE GROWTH OF TRANSPLANTED TUMOURS IN MICE AFTER CHRONIC INHALATION OF FRESH CIGARETTE SMOKE. CITATION: BR. J. CANCER 30:459-462 (1974) STUDY DESIGN: BALB/c and C57BL mice were exposed for 7-8 min on weekdays to fresh cigarette smoke in a Hamburg Ih The Machine was set to give a 1:7 smoke/air ratio. C57BL mice were exposed to fresh cigarette smoke for 3 days, 23 weeks and 38 weeks before the subcutaneous inoculation of 10e Lewis lung tumour cells. Cigarette smoke exposure was continued during tumour growth. In another study, BALB/c were exposed to fresh cigarette smoke for 20 weeks befoe the introduction of 105 TKL5 cells into the trachea. Another group of BALB/c mice were exposed to smoke for 31 weeks before receiving the intratracheal inoculation of TKL5 cells. RESULTS/FINDING: The subcutaneous growth of the Lewis lung tumour in C57BL chronically exposed to fresh cigarette smoke was increased above that in age-matched control mice. When murine sarcoma virus (Harvey) induced tumour cells were introduced to lungs of groups of BALB/c mice, only mice chronically exposed to fresh cigarette smoke died with tumour cells in the lungs. Tumour cell growth in mice during short term cigarette smoke exposure was indistinguishable from that in the control. CONCULSIONS: The results show that the subcutaneous growth of the transplantable Lewis lung tumour in mice was increased by chronic cigarette smoke inhalation. The mice chronically exposed smoke had an increased number of metastases in the lung but his was not statistically significant. However, MSV-H tumour cells introduced directly to the lungs were able to persist and develop only in the lungs of mice chronically exposed to smoke. Short term smoke inhalation did not produce these effects,